CAACGAACTATCCACCTTCAC(5251-5451)
AGCCTTCTCCTAAAGTTTCCA(5345-5366)
Primer 1
--------
Primer: CAACGAACTATCCACCTTCAC
Length: 21
%GC: 47.6
Tm: 57.2 deg C
1 礸 of primer: 159.770 pMole ends
Self-annealing: maximum score = 7
CAACGAACTATCCACCTTCAC
|||
CACTTCCACCTATCAAGCAAC
3' end score = 3
CAACGAACTATCCACCTTCAC
|
CACTTCCACCTATCAAGCAAC
Primer 2
--------
Primer: AGCCTTCTCCTAAAGTTTCCA
Length: 21
%GC: 42.9
Tm: 57.3 deg C
1 礸 of primer: 158.831 pMole ends
Self-annealing: maximum score = 10
AGCCTTCTCCTAAAGTTTCCA
||::||
ACCTTTGAAATCCTCTTCCGA
3' end score = 2
AGCCTTCTCCTAAAGTTTCCA
|
ACCTTTGAAATCCTCTTCCGA
Primer-primer
-------------
Tm diff: 0.1 deg C
Annealing: maximum score = 10
CAACGAACTATCCACCTTCAC
:||||
ACCTTTGAAATCCTCTTCCGA
3' end score = 5
CAACGAACTATCCACCTTCAC
||
ACCTTTGAAATCCTCTTCCGA
TGTCTGGAGGACCGAAACC(4021)
GTCACCAAAGAGCCACTTGC(4707)
Primer 1
--------
Primer: TGTCTGGAGGACCGAAACC
Length: 19
%GC: 57.9
Tm: 61.1 deg C
1 礸 of primer: 171.830 pMole ends
Self-annealing: maximum score = 12
TGTCTGGAGGACCGAAACC
||::||
CCAAAGCCAGGAGGTCTGT
3' end score = 6
TGTCTGGAGGACCGAAACC
||
CCAAAGCCAGGAGGTCTGT
Primer 2
--------
Primer: GTCACCAAAGAGCCACTTGC
Length: 20
%GC: 55.0
Tm: 60.8 deg C
1 礸 of primer: 165.238 pMole ends
Self-annealing: maximum score = 7
GTCACCAAAGAGCCACTTGC
|||
CGTTCACCGAGAAACCACTG
3' end score = 6
GTCACCAAAGAGCCACTTGC
||
CGTTCACCGAGAAACCACTG
Primer-primer
-------------
Tm diff: 0.2 deg C
Annealing: maximum score = 8
TGTCTGGAGGACCGAAACC
|||
CGTTCACCGAGAAACCACTG
3' end score = 4
TGTCTGGAGGACCGAAACC
|:
CGTTCACCGAGAAACCACTG
